Expression and Characterization of a Small, Xylan/Cellulose-degrading GH43 Protein Derived from Biofertilizer Metagenome
نویسندگان
چکیده
Background: A putative glycosyl hydrolase gene biof1_09 was identified from a metagenomic fosmid library of local biofertilizers in previous report [1]. The is renamed as gh43kk this study. Methods: , encoding ?-D-xylosidase amplified by polymerase chain reaction (PCR) and successfully cloned expressed Escherichia coli . recombinant protein purified metal affinity chromatography. Its properties were initially verified enzyme assay thin layer chromatography (TLC). Results: showed the highest catalytic activities at acidic pH 4 50°C toward beechwood xylan, followed carboxymethylcellulose (CMC). TLC analysis indicated release xylose glucose when xylan CMC treated with Gh43kk protein, respectively, whereas cellobiose detected avicel, cellulose filter paper used substrates, suggesting its dual function xylanase cellulase activity. great stability temperature between 10 to 50 °C wide range 8. Enzyme activity enhanced presence magnesium manganese ions, while calcium Ethylenediaminetetraacetic acid (EDTA) sodium dodecyl sulfate (SDS) inhibited Conclusion: These results suggest that could be potential candidate for application various bioconversion processes.
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ژورنال
عنوان ژورنال: The Open Microbiology Journal
سال: 2022
ISSN: ['1874-2858']
DOI: https://doi.org/10.2174/18742858-v16-e221020-2022-17